Bioremoval of hazardous cobalt, nickel, chromium, copper and cadmium compounds from contaminated soil by Nicotiana tabacum plants and associated microbiome

Contamination of soils with heavy metals leads to reduction soil fertility, destruction natural ecosystems and detrimental effects on the health society by increasing content in food chains from microorganisms plants, animals humans. Bioremediation is one most promising cost-effective methods cleaning polluted toxic metals. According current researchers, plants have genetic potential remove contaminated sites. The method thermodynamic prediction was used theoretically substantiate mechanisms interaction our prediction, exometabolite chelators anaerobic may increase mobility thereby contribute active transport their accumulation plants. Plants Nicotiana tabacum L. Djubek cultivar were as plant material for investigation. examined toxicants metals, namely cobalt (II), nickel chromium (VI), copper (II) cadmium (II). aqueous solutions metal salts added boxes after two months growing final super-high concentration – 500 mg/kg absolutely dry weight soil. Quantitative assessments chromium-resistant made cultivation agar nutrient medium NA a gradient Cu(II) Cr(VI). (leaves, stems roots) determined atomic absorption method. study revealed that inhibited growth tobacco This expressed necrosis tissues and, ultimately, complete death. Despite this, all investigated accumulated during 3–7 days before death uptake observed parts leaves, roots. highest concentrations Co(II), Ni(II), Cd(II), Cr(VI) found results show bioremoval efficiency ranged 0.60–3.65%. Given initial each (500 mg/kg), removal also high. Cadmium Thus, basic points prognosis possibility phytomicrobial consortium experimentally confirmed example N. metal-resistant microorganisms. demonstrated despite high concentration, rate damage are inplant extremely hight concentrations. Soil adaptation potencial In conditions, presumably mobilize which later absorbed obtained basis development environmental biotechnologies compounds.